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THUNDER™ Total c-Met TR-FRET Cell Signaling Assay Kit

THUNDER™ Total c-Met TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-METT Reactivity: Human Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Host Mouse
Antigen Met
Reactivity Human
Components


Eu-Total MET
Acc-Total MET
MET control lysate
5X Lysis Buffer 5
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total c-Met in cell lysates. This kit can be used to normalize data obtained with the Phospho-c-Met (Y1234/Y1235) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho c-Met (Y1234-Y1235) in A431 cells
Data show that treatment of A431 cells with HGF stimulates phosphorylation of c-Met at Y1234/Y1235 but does not affect the levels of total c-Met.
Inhibition of Phospho c-Met (Y1234-Y1235) in Hela cells
Data show that treatment of HeLa cells with PHA-665752 inhibits phosphorylation of c-Met at Y1234/Y1235 by HGF, but does not affect the levels of total c-Met.
Hela control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.