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THUNDER™ Phospho-TBK1 (S172) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-TBK1 (S172) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-TBK1P Reactivity: Human Conjugation: Brand: Bioauxilium
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Regular price $409 USD
Regular price Sale price $409 USD
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Product Details

Product Specification


Host Mouse
Antigen TBK1
Reactivity Human
Components


Eu-phospho-TBK1
Acc-phospho-TBK1
TBK1 control lysate
5X Lysis Buffer 2
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail

Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-TBK1/NAK (S172) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Data show that treatment of MCF7 cells with Calyculin A stimulates phosphorylation of TBK1/NAK at S172.
Data show that treatment of MCF7 cells with BX-795 inhibits phosphorylation of TBK1/NAK at S172 by Calyculin A.
The Z’-factor value of 0.70 indicates that the assay is robust and suitable for HTS.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
Data show the robustness of the assay following overnight incubation.