Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | TBK1 |
| Reactivity | Human |
| Components |
|
| Product Description | Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-TBK1/NAK (S172) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. |
Picture
Picture

Data show that treatment of MCF7 cells with Calyculin A stimulates phosphorylation of TBK1/NAK at S172.

Data show that treatment of MCF7 cells with BX-795 inhibits phosphorylation of TBK1/NAK at S172 by Calyculin A.

The Z’-factor value of 0.70 indicates that the assay is robust and suitable for HTS.

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.

Data show the robustness of the assay following overnight incubation.
