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THUNDER™ Phospho-SYK (Y525/Y526) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-SYK (Y525/Y526) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-SYKP Reactivity: Human, Mouse Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Host null
Antigen SYK
Reactivity Human, Mouse
Components


Eu-phospho-SYK
Acc-phospho-SYK
SYK control lysate
5X Lysis Buffer 3
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-SYK (Y525/Y526) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Product citation: Society for Neuroscience 2024: PSTR058

Picture

Stimulation of Phospho-SYK (Y525/Y526) in Raji cells
Data show that treatment of Raji cells with pervanadate stimulates phosphorylation of SYK at Y525/Y526
Inhibition of Phospho-SYK (Y525/Y526) in U-937 cells
Data show that treatment of U-937 cells with BIIB-057 inhibits phosphorylation of SYK at Y525/Y526 by pervanadate
Z'-factor determination in Raji cells
The Z’-factor value of 0.82 indicates that the assay is robust and suitable for HTS
U-937 control lysate titration (QC Test)
Data show a linear relationship between lysates pre-diluted at 1:4 or more and TR-FRET ratio values