THUNDER™ Phospho-STAT6 (Y641) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-STAT6P Reactivity: Human, Mouse Conjugation: Brand: Bioauxilium

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Product Details

Product Specification

Host	Mouse, E.coli
Antigen	STAT6
Reactivity	Human, Mouse
Components	Eu-phospho-STAT6 Acc-phospho-STAT6 STAT6 control lysate 5X Lysis Buffer 2 10X TR-FRET Detection buffer 100X Phosphatase Inhibitor Cocktail
Product Description	Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT6 (Y641) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. BEST SELLER Note: a new and improved version of this kit is available: High Performance Phospho-STAT63 (Y641) Assay Kit Product citation: PubMed 34570089

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Stimulation of Phospho-STAT6 (Y641) in HeLa cells

Data show that treatment of HeLa cells with IL-4 stimulates phosphorylation of STAT6 at Y641, but does not affect the levels of total STAT6

Inhibition of Phospho-STAT6 (Y641) in HeLa cells

Data show that treatment of HeLa cells with JAK Inhibitor 1 inhibits phosphorylation of STAT6 at Y641 by IL-4, but does not affect the levels of total STAT6