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THUNDER™ Phospho-STAT5 (Y694/Y699) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-STAT5 (Y694/Y699) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-STAT5P Reactivity: Human, Mouse Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Host Mouse
Antigen STAT5
Reactivity Human, Mouse
Components


Eu-phospho-STAT5
Acc-phospho-STAT5
STAT5 control lysate
5X Lysis Buffer 2
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT5 (Y694) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. BEST SELLER

Product citation: PubMed 37698877; PubMed 34570089

Picture

Stimulation of Phospho-STAT5 (Y694/Y699) in A431 cells
Data show that treatment of A431 cells with EGF stimulates phosphorylation of STAT5 at Y694/Y699.
Inhibition of Phospho-STAT5 (Y694/Y699) in A431 cells
Data show that treatment of A431 cells with Erlotinib inhibits phosphorylation of STAT5 at Y694/Y699 by EGF
Z'-factor determination in A431 cells
The Z’-factor value of 0.78 indicates that the assay is robust and suitable for HTS
A431 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR FRET ratio values