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THUNDER™ Phospho-STAT4 (Y693) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-STAT4 (Y693) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-STAT4P Reactivity: Human Conjugation: Brand: Bioauxilium
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Regular price $409 USD
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Product Details

Product Specification


Host Mouse
Antigen STAT4
Reactivity Human
Components


Eu-phospho-STAT4
Acc-phospho-STAT4
STAT4 control lysate
5X Lysis Buffer 2
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail

Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT4 (Y693) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. FIRST TO MARKET

Product citation: PubMed 34570089

Picture

Data show that treatment of B lymphocytes with IFNα2b stimulates phosphorylation of STAT4 at Y693.
Data show that treatment of B lymphocytes with JAK Inhibitor 1 inhibits phosphorylation of STAT4 at Y693 by IFNα2b.
Data show that stimulation of phospho-STAT4 (Y693) in B lymphocytes can be detected using the 1-plate assay protocol.
Data show a linear relationship between lysate dilutions and TR-FRET ratio value.