THUNDER™ Phospho-STAT3 (Y705) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-STAT3P Reactivity: Human, Mouse Conjugation: Brand: Bioauxilium

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$409 USD

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Product Details

Host	Mouse
Antigen	STAT3
Reactivity	Human, Mouse
Components	Eu-phospho-STAT3 Acc-phospho-STAT3 STAT3 control lysate 5X Lysis Buffer 2 10X TR-FRET Detection buffer 100X Phosphatase Inhibitor Cocktail
Product Description	Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT3 (Y705) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. BEST SELLER Note: the Phospho-STAT3 (Y705) Assay Kit uses a new antibody pair that significantly improves the assay performance. Assay benchmarking of THUNDER® Phospho-STAT3 against HTRF® and LANCE® Ultra is shown in the updated Application Note. The THUNDER® assay outperforms the two other TR-FRET assays available (HTRF® and LANCE® Ultra). Note: a new and improved version of this kit is available: High Performance Phospho-STAT3 (Y705) Assay Kit Product citation: PubMed 34570089

Data show the superior performance of the new Phospho-STAT3 (Y705) Assay compared to the previous version of the assay

Data show that treatment of HeLa cells with IFNα stimulates phosphorylation of STAT3 at Y705

Data show that treatment of HeLa cells with JAK Innibitor 1 inhibits phosphorylation of STAT3 at Y705 by IFNα

The Z’-factor value of 0.73 indicates that the assay is robust and suitable for HTS

Data show a linear relationship between lysate dilutions and TR-FRET ratio values

The THUNDER assay shows the highest S/B ratios between 1 and 4 hours of incubation

Company A (HTRF) assay shows a comparable S/B ratio to the THUNDER assay only after overnight incubation

Company B (LANCE Ultra) assay shows the lowest S/B ratios at all incubation times

Data show that treatment of NIH/3T3 cells with reference cytokines stimulates phosphorylation of STAT3 at Y705.