THUNDER™ Phospho-STAT1 (Y701) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-STAT1P Reactivity: Human Conjugation: Brand: Bioauxilium

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$409 USD

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Product Details

Host	Mouse
Antigen	STAT1
Reactivity	Human
Components	Eu-phospho-STAT1 Acc-phospho-STAT1 STAT1 control lysate 5X Lysis Buffer 1 10X TR-FRET Detection buffer 100X Phosphatase Inhibitor Cocktail
Product Description	Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT1 (Y701) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. DISCONTINUED Note: the Phospho-STAT1 (Y701) Assay Kit uses a new antibody pair that significantly improves the assay performance. Note: a new and improved version of this kit is available: High Performance Phospho-STAT1 (Y701) Assay Kit Product citation: PubMed 34570089

Data show the superior performance of the new Phospho-STAT1 (Y701) Assay compared to the previous version of the assay.

Data show that treatment of B cells with IFNα2b stimulates phosphorylation of STAT1 at Y701.

Data show that treatment of HeLa cells with JAK Inhibitor 1 inhibits phosphorylation of STAT1 at Y701 by IFNα2b.

The Z’-factor value of 0.91 indicates that the assay in HeLa cells is robust and suitable for HTS.

Data show that treatment of B cells with IFNα2b stimulates phosphorylation of STAT1 at Y701.

Data show that treatment of B cells with JAK Inhibitor 1 inhibits phosphorylation of STAT1 at Y701 by IFNα2b.

Data show that treatment of NIH/3T3 cells with reference cytokines stimulates phosphorylation of STAT1 at Y701.