1 2

THUNDER™ Phospho-SRC (Y419) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-SRC (Y419) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-SRCP Reactivity: Human Conjugation: Brand: Bioauxilium
Share:
Price:
$409 USD
$409 USD
Size:

For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Host Mouse
Antigen SRC
Reactivity Human
Components


Eu-phospho-SRC
Acc-phospho-SRC
SRC control lysate
5X Lysis Buffer 2
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-SRC (Y419) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-SRC (Y419) in A431 cells
Data show that treatment of A431 cells with H2O2 stimulates phosphorylation of SRC at Y419
Inhibition of Phospho-SRC (Y419) in A431 cells
Data show that treatment of A431 cells with Bosutinib inhibits phosphorylation of SRC at Y419 by H2O2
Z'-factor determination in A431 cells
The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS
A431 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values