THUNDER™ Phospho-SMAD2 (S465/S467) TR-FRET Cell Signaling Assay Kit
THUNDER™ Phospho-SMAD2 (S465/S467) TR-FRET Cell Signaling Assay Kit
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Product Details
Product Details
Product Specification
| Host | null |
| Antigen | SMAD2 |
| Reactivity | Human |
| Components |
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| Product Description |
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-SMAD2 (S465/S467) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. Product Citation: PubMed 35664796
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Data show that treatment of HeLa cells with TGFβ stimulates phosphorylation of SMAD2 at S465/S467
Data show that treatment of HeLa cells with SB 431542 inhibits phosphorylation of SMAD2 at S465/S467 by TGFβ
The Z’-factor value of 0.65 indicates that the assay is robust and suitable for HTS
The THUNDER assay shows a comparable S/B ratio and EC50 value to the competitor TR-FRET assay after 2h of incubation (80,000 cells/well).
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
