THUNDER™ Phospho-SLP-76 (S376) TR-FRET Cell Signaling Assay Kit
THUNDER™ Phospho-SLP-76 (S376) TR-FRET Cell Signaling Assay Kit
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$409 USD
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | SLP-76 |
| Reactivity | Human |
| Components |
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| Product Description |
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-SLP-76 (S376) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. Note: the Phospho-SLP-76 (S376) Assay Kit uses a new antibody pair that significantly improves the assay performance. Assay benchmarking is shown in the updated Application Note. The THUNDER® assay outperforms HTRF® and matches LANCE® Ultra. |
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The THUNDER assay shows a higher S/B ratio than Company A assay
The THUNDER and Company B assays show comparable S/B ratios
Data show that treatment of Jurkat cells with H2O2 stimulates phosphorylation of SLP-76 at S376
The Z’-factor value of 0.92 indicates that the assay is robust and suitable for HTS
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
Data show that stimulation of phospho-SLP-76 (S376) by H2O2 in Jurkat cells can be detected using the 1-plate assay protocol in both LV 384 and HA 96-well plates
