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THUNDER™ Phospho-RPS6 (S235/S236) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-RPS6 (S235/S236) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-RPS6AP Reactivity: Human Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Host Mouse
Antigen Ribosomal Protein S6
Reactivity Human
Components


Eu-phospho-RPS6S235
Acc-phospho-RPS6S235
RPS6 control lysate
5X Lysis Buffer 4
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-Ribosomal Protein S6 (S235/S236) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-RPS6 (S235/S236) in MCF7 cells
Data show that treatment of MCF7 cells with insulin stimulates phosphorylation of RPS6 at S235/S236
inhibition of Phospho-RPS6 (S235/S236) in MCF7 cells
Data show that treatment of MCF7 cells with LY294002 inhibits phosphorylation of RPS6 at S235/S236 by insulin
Z'-factor determination in MCF7 cells
The Z’-factor value of 0.52 indicates that the assay is robust and suitable for HTS
MCF& control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values