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THUNDER™ Phospho-Rb (S807/S811) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-Rb (S807/S811) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-RBS807P Reactivity: Human Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Host Mouse
Antigen RB
Reactivity Human
Components


Eu-phospho-Rb-S807
Acc-phospho-Rb-S807
Rb control lysate
5X Lysis Buffer 3
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-Rb (S807/S811) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Inhibition of Phospho-Rb-S807 in HT-29 cells
Data show that treatment of HT-29 cells with palbociclib inhibits phosphorylation of Rb at S807/S811.
Z'-factor of Phospho-Rb (S807) in Jurkat cells
The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS.
HT-29 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.