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THUNDER™ Phospho-p70 S6K (T389) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-p70 S6K (T389) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-P70S6KP Reactivity: Human Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Host Mouse
Antigen p70 S6K
Reactivity Human
Components


Eu-phospho-P70S6
Acc-phospho-P70S6
P70S6 control lysate
5X Lysis Buffer 3
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p70 S6K (T389) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-P70 S6K (T389) in MCF7 cells
Data show that treatment of MCF7 cells with Insulin stimulates phosphorylation of p70 S6K at T389
Inhibition of Phospho-P70 S6K (T389) in MCF7 cells
Data show that treatment of MCF7 cells with LY294002 inhibits phosphorylation of p70 S6K at T389 by insulin
Z'-factor determination in MCF7 cells
The Z’-factor value of 0.6 indicates that the assay is robust and suitable for HTS
MCF7 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values