THUNDER™ Phospho-p70 S6K (T389) + Total p70 S6K TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-P70S6KPT Reactivity: Human Conjugation: Brand: Bioauxilium

Price:

$1,375 USD

Size: 500points

Quantity:

For shipping services or bulk orders, you may request a quotation.

Secure checkout with

View full details

Product Details

Product Specification

Antigen	p70 S6K
Reactivity	Human
Components	400 points for phospho; 100 points for total Eu-phospho-P70S6 Acc-phospho-P70S6 Eu-Total P70S6 Acc-Total P70S6 P70S6 control lysate 5X Lysis Buffer 3 10X TR-FRET Detection buffer 100X Phosphatase Inhibitor Cocktail
Product Description	Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p70 S6K (T389) and Total p70 S6K in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total p70 S6K proteins from the same lysate sample in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-P70 S6K (T389) in MCF7 cells

. Data show that treatment of MCF7 cells with Insulin stimulates phosphorylation of p70 S6K at T389, but does not affect the levels of total p70 S6K

Data show that treatment of MCF7 cells with LY294002 inhibits phosphorylation of p70 S6K at T202/Y204 by insulin, but does not affect the levels of total p70 S6K

MCF7 control lysate titration (QC Test) Phospho-P70 S6K (T389)

Data show a linear relationship between lysate dilutions and TR-FRET ratio values

MCF7 control lysate titration (QC Test) total P70 S6K

Data show a linear relationship between lysate dilutions and TR-FRET ratio values

Item added to your cart

THUNDER™ Phospho-p70 S6K (T389) + Total p70 S6K TR-FRET Cell Signaling Assay Kit