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THUNDER™ Phospho-p53 (S15) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-p53 (S15) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-P53P Reactivity: Human Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Host Mouse
Antigen p53
Reactivity Human
Components


Eu-phospho-p53
Acc-phospho-p53
p53 control lysate
5X Lysis Buffer 2
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p53 (S15) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-P53 (S15) in HEK293 cells
Data show that treatment of HEK293 cells with Etoposide stimulates phosphorylation of p53 at Ser15
Z'-factor determination in HEK293 cells
The Z’-factor value of 0.6 indicates that the assay is robust and suitable for HTS
HEK293 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TRFRET ratio values