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THUNDER™ Phospho-p53 (S15) + Total p53 TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-p53 (S15) + Total p53 TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-P53PT Reactivity: Human Conjugation: Brand: Bioauxilium
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Regular price $1,375 USD
Regular price Sale price $1,375 USD
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Product Details

Product Specification


Antigen p53
Reactivity Human
Components

400 points for phospho; 100 points for total


Eu-phospho-p53
Acc-phospho-p53
Eu-Total p53
Acc-Total p53
p53 control lysate
5X Lysis Buffer 2
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail

Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p53 (S15) and Total p53 in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total p53 proteins in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-P53 (S15) in HEK293 cells
Data show that treatment of HEK293 cells with Etoposide stimulates phosphorylation of p53 at Ser15, but does not affect the levels of total p53
HEK293 control lysate titration (QC Test) Phospho-P53(S15)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
HEK293 control lysate titration (QC Test) Total P53
Data show a linear relationship between lysate dilutions and TR-FRET ratio values