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THUNDER™ Phospho-p38αβγ MAPK (T180/Y182) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-p38αβγ MAPK (T180/Y182) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-P38P Reactivity: Human Conjugation: Brand: Bioauxilium
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Regular price $409 USD
Regular price Sale price $409 USD
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Product Details

Product Specification


Host Mouse
Antigen p38αβγ MAPK
Reactivity Human
Components


Eu-phospho-p38
Acc-phospho-p38
p38a control lysate
5X Lysis Buffer 6
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail

Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p38αβγ MAPK14/11/12 (T180/Y182) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. Assay benchmarking is shown in the updated Application Note. The THUNDER® assay outperforms the other TR-FRET assay available (HTRF®).

Picture

Stimulation of Phospho-p38αβγ (T180-Y182) in HeLa cells
Data show that treatment of HeLa cells with Anisomycin stimulates phosphorylation of p38αβγ at T180/Y182.
Z'-factor determination in HeLa cells
The Z’-factor value of 0.73 indicates that the assay is robust and suitable for HTS.
HeLa Control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.