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THUNDER™ Phospho-p38α MAPK (T180/Y182) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-p38α MAPK (T180/Y182) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-P38AP Reactivity: Human Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Host Mouse
Antigen p38α MAPK
Reactivity Human
Components


Eu-phospho-p38a
Acc-phospho-p38a
p38a control lysate
5X Lysis Buffer 6
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p38α MAPK14 (T180/Y182) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-p38α (T180-Y182) in HeLa cells
Data show that treatment of HeLa cells with Anisomycin stimulates phosphorylation of p38α at T180/Y182
Z'-factor determination in HeLa cells
The Z’-factor value of 0.71 indicates that the assay is robust and suitable for HTS.
HeLa Control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.