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THUNDER™ Phospho-MEK1 (S218/S222) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-MEK1 (S218/S222) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-MEK1P Reactivity: Human Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Host null
Antigen MEK1
Reactivity Human
Components


Eu-phospho-MEK1
Acc-phospho-MEK1
MEK1 control lysate
5X Lysis Buffer 3
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-MEK1 (S218/S222) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-MEK1 (S218-S222) in HeLa cells
Data show that treatment of HeLa cells with PMA stimulates phosphorylation of MEK1 at S218/S222
Inhibition of Phospho-MEK1 (S218-S222) in HeLa cells
Data show that treatment of HeLa cells with L779,450 inhibits phosphorylation of MEK1 at S218/S222 by PMA.
Z'-factor determination in HeLa cells
The Z’-factor value of 0.76 indicates that the assay is robust and suitable for HTS.
Hela control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
All-in-one-well assay in HeLa cells
Data show that stimulation of phospho-MEK1 (S218/S222) in HeLa cells can be detected using the 1-plate assay protocol.