Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | LCK |
| Reactivity | Human |
| Components |
|
| Product Description | Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-LCK (Y394) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. |
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Data show that treatment of Jurkat cells with Pervanadate stimulates phosphorylation of LCK at Y394.

The Z’-factor value of 0.72 indicates that the assay is robust and suitable for HTS.

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.

Lysates were generated with the corresponding kit's Lysis Buffer. Data show that the THUNDER Assay Kit generates superior S/B ratios compared to the Competitor's assay.
