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THUNDER™ Phospho-LCK (Y394) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-LCK (Y394) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-LCKP Reactivity: Human Conjugation: Brand: Bioauxilium
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Regular price $409 USD
Regular price Sale price $409 USD
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Product Details

Product Specification


Host Mouse
Antigen LCK
Reactivity Human
Components


Eu-phospho-LCK
Acc-phospho-LCK
LCK control lysate
5X Lysis Buffer 2
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail

Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-LCK (Y394) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Data show that treatment of Jurkat cells with Pervanadate stimulates phosphorylation of LCK at Y394.
The Z’-factor value of 0.72 indicates that the assay is robust and suitable for HTS.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
Phospho-LCK benchmarking
Lysates were generated with the corresponding kit's Lysis Buffer. Data show that the THUNDER Assay Kit generates superior S/B ratios compared to the Competitor's assay.