THUNDER™ Phospho-Insulin Receptor β (Y1150/Y1151) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-INRP Reactivity: Human Conjugation: Brand: Bioauxilium

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$409 USD

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Product Details

Host	Mouse, E.coli
Antigen	Insulin Receptor β
Reactivity	Human
Components	Eu-phospho-INR Acc-phospho-INR INR control lysate 5X Lysis Buffer 4 10X TR-FRET Detection buffer 100X Phosphatase Inhibitor Cocktail
Product Description	Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-Insulin Receptor β (Y1150/Y1151) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Data show that treatment of B lymphocytes with insulin stimulates phosphorylation of IRβ at Y1150/Y1151

Data show that treatment of B lymphocytes with S961 inhibits phosphorylation of IRβ at Y1150/Y1151 by insulin

The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS

Data show a linear relationship between lysate dilutions and TR-FRET ratio values

Data show that stimulation of phospho-IRβ at Y1150/Y1151 can be detected using the 1-plate assay protocol.