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THUNDER™ Phospho-ERK1/2 (T202/Y204) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-ERK1/2 (T202/Y204) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-ERKP Reactivity: Human, Mouse Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Host Mouse
Antigen ERK1/2
Reactivity Human, Mouse
Components


Eu-phospho-ERK
Acc-phospho-ERK
ERK control lysate
5X Lysis Buffer 1
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-ERK1/2 (T202/Y204) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. Assay benchmarking is shown in the updated Application Note. The THUNDER® assay outperforms the two other TR-FRET assays available (HTRF® and LANCE® Ultra). BEST SELLER

Picture

Stimulation of PHOSPHO-ERK1-2 (T202-Y204) in HEK293 cells
Data show that treatment of HEK293 cells with EGF stimulates phosphorylation of ERK1/2 at T202/Y204.
Inhibition of Phospho-ERK1-2 (T202-Y204) in HEK293 cells
Data show that treatment of HEK293 cells with Erlotinib inhibits phosphorylation of ERK1/2 at T202/Y204 by EGF.
Z'-factor determination (QC Test) HEK293 cells
The Z’-factor value of 0.6 indicates that the assay is robust and suitable for HTS.
HEK293 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
Head-to-head kit comparison
Data show a comparable performance between THUNDER™ and the competing TR-FRET kit.
Head-to-head kit comparison
Data show that THUNDER™ outperforms the competing TR-FRET kit.