THUNDER™ Phospho-eIF4E (S209) TR-FRET Cell Signaling Assay Kit
THUNDER™ Phospho-eIF4E (S209) TR-FRET Cell Signaling Assay Kit
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Product Details
Product Details
Product Specification
| Host | null |
| Antigen | elF4E |
| Reactivity | Human |
| Components |
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| Product Description | Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-eIF4E (S209) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. DISCONTINUED |
Picture
Picture
Data show that treatment of HEK293 cells with CGP 57380 inhibits phosphorylation of eIF4E at S209 by EGF.
Data show that treatment of HeLa cells with CGP 57380 or PP242 inhibits phosphorylation of eIF4E at S209 by EGF.
The Z’-factor value of 0.68 indicates that the assay is robust and suitable for HTS.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
