1 2

THUNDER™ Phospho-eIF2α (S51) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-eIF2α (S51) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-EIF2aP Reactivity: Human Conjugation: Brand: Bioauxilium
Share:
Price:
$409 USD
$409 USD
Size:

For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Host Mouse
Antigen elF2α
Reactivity Human
Components


Eu-phospho-EIF2α
Acc-phospho-EIF2α
EIF2α control lysate
5X Lysis Buffer 1
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-EIF2α (S51) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-eIF2α (S51) in Hela cells
Data show that treatment of HeLa cells with Calyculin A stimulates phosphorylation of eIF2α at S51.
Z'-factor determination in Hela cells
The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS.
Hela Control cell lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.