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THUNDER™ Phospho-eIF2α (S51) + Total eIF2α TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-eIF2α (S51) + Total eIF2α TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-EIF2aPT Reactivity: Human Conjugation: Brand: Bioauxilium
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$1,375 USD
$1,375 USD
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Product Details

Product Specification


Antigen elF2α
Reactivity Human
Components

400 points for phospho; 100 points for total


Eu-phospho-EIF2α
Acc-phospho-EIF2α
Eu-Total EIF2α
Acc-Total EIF2α
EIF2α control lysate
5X Lysis Buffer 1
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-EIF2α (S51) and Total eIF2α in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total eIF2α proteins from the same lysate sample in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-eIF2a (S51) in Hela cells
Data show that treatment of HeLa cells with Calyculin A stimulates phosphorylation of eIF2α at S51 but does not affect the levels of total eIF2α.
Hela control lysate-titration (QC Test) PHOSPHO-eIF2α (S51)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
Hela control lysate titration (QC Test) TOTAL-eIF2a
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.