THUNDER™ Phospho-EGFR (Y1068) TR-FRET Cell Signaling Assay Kit
THUNDER™ Phospho-EGFR (Y1068) TR-FRET Cell Signaling Assay Kit
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | EGFR |
| Reactivity | Human |
| Components |
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| Product Description |
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-EGFR (Y1068) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. BEST SELLER Product citation: PubMed 40452841 |
Picture
Picture
Data show that treatment of A431 cells with EGF stimulates phosphorylation of EGFR at Y1068.
Data show that treatment of A431 cells with Cetuximab inhibits phosphorylation of EGFR at Y1068 by EGF.
The Z’-factor value of 0.73 indicates that the assay is robust and suitable for HTS.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
