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THUNDER™ Phospho-EGFR (Y1068) + Total EGFR TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-EGFR (Y1068) + Total EGFR TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-EGFRPT Reactivity: Human Conjugation: Brand: Bioauxilium
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$1,375 USD
$1,375 USD
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Product Details

Product Specification


Antigen EGFR
Reactivity Human
Components

400 points for phospho; 100 points for total


Eu-phospho-EGFR
Acc-phospho-EGFR
Eu-Total EGFR
Acc-Total EGFR
EGFR control lysate
5X Lysis Buffer 1
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-EGFR (Y1068) and Total EGFR in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total EGFR proteins from the same lysate sample in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-EGFR (Y1068) in A431 cells
Data show that treatment of A431 cells with EGF stimulates phosphorylation of EGFR at Y1068 but does not have a major effect on the levels of total EGFR.
Inhibition of Phospho-EGFR (Y1068) in A431 cells
Data show that treatment of A431 cells with Cetuximab inhibits phosphorylation of EGFR at Y1068 by EGF but does not have a major effect on the levels of total EGFR.
A431 control lysate titration (QC Test)Phospho-EGFR (Y1068)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
A431 control lysate titration(QC Test) Total EGFR
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.