1 2

THUNDER™ Phospho-CREB (S133) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-CREB (S133) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-CREBP Reactivity: Human, Mouse Conjugation: Brand: Bioauxilium
Share:
Price:
$409 USD
$409 USD
Size:

For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Host Mouse
Antigen CREB
Reactivity Human, Mouse
Components


Eu-phospho-CREB
Acc-phospho-CREB
CREB control lysate
5X Lysis Buffer 2
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-CREB (S133) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho CREB (S133) in NIH3T3 cells
Data show that treatment of NIH3T3 cells with Forskolin stimulates phosphorylation of CREB at S133.
Inhibition of Phospho CREB (S133) in A431 cells
Data show that treatment of A431 cells with Erlotinib inhibits phosphorylation of CREB at S133 by EGF.
Z'-factor determination in A431 cells
The Z’-factor value of 0.61 indicates that the assay is robust and suitable for HTS.
B lymphocyte control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.