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THUNDER™ Phospho-CREB (S133) + Total CREB TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-CREB (S133) + Total CREB TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-CREBPT Reactivity: Human, Mouse Conjugation: Brand: Bioauxilium
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$1,375 USD
$1,375 USD
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Product Details

Product Specification


Host Mouse
Antigen CREB
Reactivity Human, Mouse
Components

400 points for phospho; 100 points for total


Eu-phospho-CREB
Acc-phospho-CREB
Eu-Total CREB
Acc-Total CREB
CREB control lysate
5X Lysis Buffer 2
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-CREB (S133) and Total CREB in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total CREB proteins from the same lysate sample in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho CREB (S133) in NIH3T3 cells
Data show that treatment of NIH3T3 cells with Forskolin stimulates phosphorylation of CREB at S133 but does not affect the levels of total CREB.
Inhibition of Phospho CREB (S133) in A431 cells
Data show that treatment of A431 cells with Erlotinib inhibits phosphorylation of CREB at S133 by EGF, but does not affect the levels of total CREB.
B lymphocyte control lysate titration (QC Test) Phospho CREB (S133)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
B lymphocyte control lysate titration (QC-test) Total CREB
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.