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THUNDER™ Phospho-c-Met (Y1234/Y1235) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-c-Met (Y1234/Y1235) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-METP Reactivity: Human Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Host Mouse
Antigen Met
Reactivity Human
Components


Eu-phospho-MET
Acc-phospho-MET
MET control lysate
5X Lysis Buffer 5
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-c-Met (Y1234/Y1235) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Product citation: PubMed 40452841

Picture

Stimulation of Phospho-c-Met (Y1234-Y1235) in A431 and Hela cells
Data show that treatment of A431 or HeLa cells with HGF stimulates phosphorylation of c-Met at Y1234/Y1235.
Inhibition of Phospho-c-Met (Y1234-Y1235) in HeLa cells
Data show that treatment of HeLa cells with PHA-665752 inhibits phosphorylation of c-Met at Y1234/Y1235 by HGF.
Z'-factor determination in HeLa cells
The Z’-factor value of 0.7 indicates that the assay is robust and suitable for HTS.
HeLa control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.