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THUNDER™ Phospho-BTK (Y223) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-BTK (Y223) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-BTKP Reactivity: Human Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Host Mouse
Antigen BTK
Reactivity Human
Components


Eu-phospho-BTK
Acc-phospho-BTK
BTK control lysate
5X Lysis Buffer 2
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-BTK (Y223) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-BTK (Y223) in Raji cells
Data show that treatment of Raji cells with Pervanadate stimulates phosphorylation of BTK at Y223
Inhibition of Phospho-BTK (Y223) in Raji cells
Data show that treatment of Raji cells with Ibrutinib inhibits phosphorylation of BTK at Y223 by Pervanadate
Z'-factor determination in Raji cells
The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS
Phospho-BTK (Y223) Control lysate titration
Data show a linear relationship between lysate dilutions and TR-FRET ratio values