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THUNDER™ Phospho-BAD (S112) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-BAD (S112) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-BADP Reactivity: Human Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Antigen BAD
Reactivity Human
Components


Eu-phospho-BAD
Acc-phospho-BAD
BAD control lysate
5X Lysis Buffer 1
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-BAD (S112) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-BAD (S112) in MCF7 cells
Data show that treatment of MCF7 cells with PMA stimulates phosphorylation of BAD at S112
Inhibition of Phospho-BAD (S112) in MCF7 cells
Data show that treatment of MCF7 cells with UCN-01 inhibits phosphorylation of BAD at S112 by PMA
Z'-factor determination in MCF7 cells
The Z’-factor value of 0.76 indicates that the assay is robust and suitable for HTS
HEK293 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values