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THUNDER™ Phospho-AKT pan (T308) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-AKT pan (T308) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-AKTT308P Reactivity: Human, Mouse Conjugation: Brand: Bioauxilium
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Regular price $409 USD
Regular price Sale price $409 USD
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Product Details

Product Specification


Host null
Antigen AKT pan
Reactivity Human, Mouse
Components


Eu-phospho-AKTT308
Acc-phospho-AKTT308
AKT control lysate
5X Lysis Buffer 1
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail

Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-AKT 1/2/3 (T308) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-AKT pan (T308) in NIH3T3 cells
Data show that treatment of NIH3T3 cells with PDGF-AA stimulates phosphorylation of AKT at T308.
Inhibition of Phospho-AKTpan(T308) in NIH3T3 cells
Data show that treatment of NIH3T3 cells with Wortmannin inhibits phosphorylation of AKT at T308 by PDGF-AA.
Z'-factor determination-NIH3T3-cells
The Z’-factor value of 0.7 indicates that the assay is robust and suitable for HTS.