THUNDER™ Phospho-AKT pan (S473) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-AKTS473P Reactivity: Human, Mouse Conjugation: Brand: Bioauxilium

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$409 USD

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Product Details

Host	Mouse
Antigen	AKT pan
Reactivity	Human, Mouse
Components	Eu-phospho-AKTS473 Acc-phospho-AKTS473 AKT control lysate 5X Lysis Buffer 7 10X TR-FRET Detection buffer 100X Phosphatase Inhibitor Cocktail
Product Description	Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-AKT1/2/3 (S473) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. Assay benchmarking is shown in the updated Application Note. The THUNDER™ assay outperforms the two other TR-FRET assays available. BEST SELLER

Treatment of NIH3T3 cells with PDGF-AA stimulates phosphorylation of AKT pan at S473.

Treatment of NIH3T3 cells with Wortmannin inhibits phosphorylation of AKT pan at S473.

The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS.

Data show a linear relationship between lysate dilutions and TR FRET ratio values.

Treatment of Jurkat cells with LY294002 inhibits phosphorylation of AKT pan at S473.

Data show that the THUNDER™ assay kit outperforms the other two competing TR-FRET kits.