THUNDER™ Phospho-AKT pan (S473) + Total AKT pan TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-AKTS473PT Reactivity: Human, Mouse Conjugation: Brand: Bioauxilium

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$1,375 USD

Size: 500points

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Product Details

Host	Mouse
Antigen	AKT pan
Reactivity	Human, Mouse
Components	400 points for phospho; 100 points for total Eu-phospho-AKTS473 Acc-phospho-AKTS473 Eu-Total AKT Acc-Total AKT AKT control lysate 5X Lysis Buffer 7 10X TR-FRET Detection buffer 100X Phosphatase Inhibitor Cocktail
Product Description	Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-AKT 1/2/3 (S473) and Total AKT 1/2/3 in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total AKT pan proteins from the same lysate sample in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Data show that treatment of NIH3T3 cells with PDGF-AA stimulates phosphorylation of AKT pan at S473 but does not affect the levels of total AKT pan.

Data show that treatment of NIH3T3 cells with Wortmannin inhibits phosphorylation of AKT pan at S473 but does not affect the levels of total AKT pan.

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.