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THUNDER™ Phospho-4EBP1 (T37/T46) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-4EBP1 (T37/T46) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-4EBP1P Reactivity: Human Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Host Mouse
Antigen 4EBP1
Reactivity Human
Components


Eu-phospho-4EBP1
Acc-phospho-4EBP1
4EBP1 control lysate
5X Lysis Buffer 2
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-4EBP1 (T37/T46) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. Assay benchmarking is shown in the updated Application Note.

Picture

Inhibition of Phospho 4EBP1 (T37-T46)in A431 cells
Treatment of A431 cells with PP242 inhibits phosphorylation of 4EBP1 at T37/T46.
Inhibition of Phospho 4EBP1 (T37-T46) in HEK293 cells
Treatment of HEK293 cells with PP242 inhibits phosphorylation of 4EBP1 at T37/T46
Z-factor determination in A431 cells
Z’-factor value of 0.73 indicates that the assay is robust and suitable for HTS
A431 control lysate titration (QC test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values