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THUNDER™ Human IL-6 TR-FRET Biomarker Assay Kit

THUNDER™ Human IL-6 TR-FRET Biomarker Assay Kit

Catalog Number: KIT-IL6 Brand: Bioauxilium
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$404 USD
$404 USD
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Product Details

Product Specification


Host Mouse
Antigen IL-6
Components


Eu-anti-IL-6
Acc-anti-IL-6
5X Assay Buffer 1
Product Description

Sandwich TR-FRET immunoassay for the quantitative measurement of human IL-6 in cell culture supernatants. The kit does not include a recombinant human IL-6 standard, which must be purchased separately from ProSpec (catalog number CYT-213). A detailed assay protocol and full assay validation can be found in the Technical Data Sheet. BEST SELLER

Key features and benefits:

  • Homogeneous: no wash, easy and rapid protocol
  • High sensitivity (15 µL sample), specificity, robustness, and reproducibility
  • High-quality antibody pairs ensure lot-to-lot consistency
  • Reliable: rigorous development, validation, and quality control testing
  • Cost-effective: 5 flexible formats; larger quantities available upon request

Picture

Typical standard curve lamp
Standard curve for the THUNDER human IL-6 TR-FRET assay run in a 384-well white plate and read on a TR-FRET enabled reader using lamp excitation.
Typical standard curve laser
Standard curve for the THUNDER human IL-6 TR-FRET assay run in a 384-well white plate and read on a TR-FRET enabled reader using laser excitation.
THUNDER Human IL-6 standard curve
A head-to-head benchmark comparison of the THUNDER Human IL-6 assay (red curve above) and a competitor TR-FRET Human IL-6 assay (blue curve on the right) was conducted. Standard curves were run using the corresponding reagents and protocols.
Competitor Human IL-6 standard curve
The THUNDER assay exhibits a wider dynamic range and a much higher S/B ratio compared to the Competitor assay. In addition, the THUNDER assay generates data faster (after 1 hour of incubation versus 2 hours).
Parallelism with PBMC cells
Supernatants from PBMC cells treated for two days with LPS and IL-2 were serially diluted with culture medium and assayed for IL-6. Recovery values between 95-114% of the neat sample demonstrate assay parallelism.