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THUNDER™ Human IL-2 TR-FRET Biomarker Assay Kit

THUNDER™ Human IL-2 TR-FRET Biomarker Assay Kit

Catalog Number: KIT-IL2 Brand: Bioauxilium
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$404 USD
$404 USD
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Product Details

Product Specification


Host Mouse
Antigen IL-2
Components


Eu-anti-IL-2
Acc-anti-IL-2
5X Assay Buffer 1
Product Description

Sandwich TR-FRET immunoassay for the quantitative measurement of human IL-2 in cell culture supernatants. The kit does not include a recombinant human IL-2 standard, which must be purchased separately from R&D Systems® (catalogue number 202-IL). A detailed assay protocol and full assay validation can be found in the Technical Data Sheet. BEST SELLER

Key features and benefits:

  • Homogeneous: no wash, easy and rapid protocol
  • High sensitivity (15 µL sample), specificity, robustness, and reproducibility
  • High-quality antibody pairs ensure lot-to-lot consistency
  • Reliable: rigorous development, validation, and quality control testing
  • Cost-effective: 5 flexible formats; larger quantities available upon request
  • Bulk orders and batch reservations available

Picture

Typical standard curve lamp
Standard curve for the human IL-2 TR-FRET assay run in a 384-well white plate and read on a TR-FRET enabled reader using lamp excitation.
Typical standard curve laser
Standard curve for the human IL-2 TR-FRET assay run in a 384-well white plate and read on a TR-FRET enabled reader using laser excitation.
Parallelism with Jurkat cells
Supernatants from Jurkat cells treated overnight with PMA and anti-CD3 were serially diluted with culture medium and assayed for IL-2. Recovery values between 101-104% of the neat sample demonstrate assay parallelism.