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THUNDER™ Human CCL2/MCP1 TR-FRET Biomarker Assay Kit

THUNDER™ Human CCL2/MCP1 TR-FRET Biomarker Assay Kit

Catalog Number: KIT-CCL2 Brand: Bioauxilium
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$404 USD
$404 USD
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Product Details

Product Specification


Host Mouse
Antigen CCL2/MCP1
Components


Eu-anti-CCL2/MCP-1
Acc-anti-CCL2/MCP-1
5X Assay Buffer 1
Product Description

Sandwich TR-FRET immunoassay for the quantitative measurement of human CCL2 (MCP-1; MCAF; JE) in cell culture supernatants. The kit does not include a recombinant human CCL2 standard, which must be purchased separately from R&D Systems® (catalog number 279-MC-010). A detailed assay protocol and full assay validation can be found in the Technical Data Sheet.

Key features and benefits:

  • Homogeneous: no wash, easy and rapid protocol
  • High sensitivity (15 µL sample), specificity, robustness, and reproducibility
  • High-quality antibody pairs ensure lot-to-lot consistency
  • Reliable: rigorous development, validation, and quality control testing
  • Cost-effective: 5 flexible formats; larger quantities available upon request

Picture

Typical standard curve lamp
Standard curve for the human CCL2/MCP1 TR-FRET assay run in a 384-well white plate and read on a TR-FRET enabled reader using lamp excitation.
Typical standard curve laser
Standard curve for the human CCL2/MCP1 TR-FRET assay run in a 384-well white plate and read on a TR-FRET enabled reader using laser excitation.
Assay performance on different readers
Assay performance on three different microplate readers.
Parallelism-CCL2-PBMC
Supernatants from PBMC cells treated for two days with LPS and IL-2 were serially diluted with culture medium and assayed for CCL-2. Recovery values between 94-114% of the 4-fold diluted sample demonstrate assay parallelism.