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THUNDER™ High Performance Phospho-STAT6 (Y641) TR-FRET Cell Signaling Assay Kit

THUNDER™ High Performance Phospho-STAT6 (Y641) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-HP-STAT6P Reactivity: Human, Mouse Conjugation: Brand: Bioauxilium
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Regular price $430 USD
Regular price Sale price $430 USD
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Product Details

Product Specification


Host null
Antigen STAT6
Reactivity Human, Mouse
Components


Eu-HP-phospho-STAT6
Acc-HP-phospho-STAT6
STAT6 control lysate
5X Lysis Buffer 8
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail

Product Description

High performance sandwich TR-FRET immunoassay for semi-quantitative detection of Human and Mouse Phospho-STAT6 (Y641) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. BEST SELLER

Note: This kit is a new and improved high performance version of catalog number KIT-STAT6P-XXX, which will remain available for purchase.

All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

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High Performance Phospho-STAT6 vs Current Kit
Data show the superior S/B ratio obtained with the High Performance Phospho-STAT6 (Y641) Kit compared to the standard version of the kit.
Stimulation of Phospho-STAT6 (Y641) in HeLa cells
Data show that treatment of HeLa cells with IL-4 stimulates phosphorylation of STAT6 at Y641. Robust S/B ratio and pharmacology can be obtained after 1h of incubation.
Inhibition of Phospho-STAT6 (Y641) in HeLa cells
Data show that treatment of HeLa cells with JAK Inhibitor 1 inhibits phosphorylation of STAT6 at Y641 by IL-4. Robust S/B ratio and pharmacology can be obtained after 1h of incubation.
Z'-factor determination in HeLa cells
The Z’-factor value of 0.86 indicates that the assay is robust and suitable for HTS.
Stimulation of Phospho-STAT6 (Y641) in THP-1 cells
Data show that treatment of THP-1 with IL-4 stimulates phosphorylation of STAT6 at Y641.
Z'-factor determination in THP-1 cells
The Z’-factor value of 0.91 indicates that the assay is robust and suitable for HTS
Stimulation of Phospho-STAT6 (Y641) in NIH/3T3 cells
Data show that the High Performance kit detects mouse Phospho-STAT6 (Y641).