THUNDER™ High Performance Phospho-STAT6 (Y641) + Total STAT6 TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-HP-STAT6PT Reactivity: Human Conjugation: Brand: Bioauxilium

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$1,348 USD

Size: 500points

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Product Details

Host	null
Antigen	STAT6
Reactivity	Human
Components	400 points for phospho; 100 points for total Eu-HP-phospho-STAT6 Acc-HP-phospho-STAT6 Eu-HP-Total STAT6 Acc-HP-Total STAT6 STAT6 control lysate 5X Lysis Buffer 8 10X TR-FRET Detection buffer 100X Phosphatase Inhibitor Cocktail
Product Description	High Performance sandwich TR-FRET immunoassay for semi-quantitative detection of Human Phospho-STAT6 (Y641) and Total STAT6 in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total STAT6 proteins from the same lysate sample in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. Note: This kit is a new and improved high performance version of catalog number KIT-STAT6PT-500, which will remain available for purchase. All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Treatment of HeLa cells with IL-4 stimulates phosphorylation of STAT6 at Y641 but does not affect the levels of Total STAT6.

Treatment of HeLa cells with JAK Inhibitor 1 inhibits phosphorylation of STAT6 at Y641 by IL-4 but does not affect the levels of Total STAT6.

Data show a linear relationship between lysate dilutions and TR-FRET ratio values for Phospho-STAT6 (Y641)

Data show a linear relationship between lysate dilutions and TR-FRET ratio values for Total STAT6

Treatment of THP-1 cells with IL-4 stimulates phosphorylation of STAT6 at Y641 but does not affect the levels of Total STAT6.

Treatment of THP-1 cells with JAK Inhibitor 1 inhibits phosphorylation of STAT6 at Y641 by IL-4 but does not affect the levels of Total STAT6.