THUNDER™ High Performance Phospho-STAT3 (Y705) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-HP-STAT3P Reactivity: Human, Mouse Conjugation: Brand: Bioauxilium

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$430 USD

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Product Details

Product Specification

Host	null
Antigen	STAT3
Reactivity	Human, Mouse
Components	Eu-HP-phospho-STAT3 Acc-HP-phospho-STAT3 STAT3 control lysate 5X Lysis Buffer 8 10X TR-FRET Detection buffer 100X Phosphatase Inhibitor Cocktail
Product Description	High performance sandwich TR-FRET immunoassay for semi-quantitative detection of Human and Mouse Phospho-STAT3 (Y705) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. BEST SELLER Note: This kit is a new and improved high performance version of catalog number KIT-STAT3P-XXX, which will remain available for purchase. See the new Application Note for a comparison of THUNDER® High Performance Phospho-STAT3 versus HTRF® and AlphaLISA SureFire® Ultra. The High-Performance THUNDER assay outperformed HTRF and matched SureFire in terms of assay sensitivity while being simpler, faster, and more cost-effective. All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

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High Performance versus Standard Phospho-STAT3 Assay Kit

Data show the superior S/B ratio obtained with the High Performance Phospho-STAT3 (Y705) Assay compared to the standard version of the assay

Stimulation of Phospho-STAT3 (Y705) in HeLa cells

Data show that treatment of HeLa cells with IFNα2b stimulates phosphorylation of STAT3 at Y705. Robust S/B ratio and pharmacology are obtained after 1 h of incubation.

Inhibition of Phospho-STAT3 (Y705) in HeLa cells

Data show that treatment of HeLa cells with JAK inhibitor 1 inhibits phosphorylation of STAT3 at Y705 by IFNα2b. Robust S/B ratio and pharmacology are obtained after 1 h of incubation.