Thrombospondin 1 Recombinant Rabbit mAb (S-4153-22)

Catalog Number: S0B60067 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	Thrombospondin 1
Synonyms	Thrombospondin-1; TSP; TSP1; THBS1
Immunogen	Synthetic Peptide
Location	Secreted, Endoplasmic reticulum
Accession	P07996
Clone Number	S-4153-22
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	mouse platelet, rat platelet
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Hu, Ms, Rt
IHC-P	1:2000	Hu, Ms, Rt

Background

Thrombospondin-1 (TSP-1) is a multifunctional secreted glycoprotein composed of trimers and belongs to the matricellular protein family within the extracellular matrix. It contains multiple domains that interact with various cell surface receptors (such as CD36, CD47, integrins) and matrix molecules (such as heparin, fibronectin, collagen). Physiologically, TSP-1 is best known as an endogenous angiogenesis inhibitor—it binds to the CD36 receptor, activating p38 MAPK and caspase signaling pathways to directly induce endothelial cell apoptosis, thereby potently suppressing angiogenesis. Additionally, TSP-1 is a key activator of transforming growth factor-beta (TGF-β), releasing active TGF-β through conformational changes to regulate immune responses, fibrosis, and tissue remodeling. Pathologically, dysregulation of TSP-1 expression is associated with various diseases: in the tumor microenvironment, its high expression can inhibit tumor angiogenesis and immune surveillance, though it may also be associated with tumor progression and metastasis. In cardiovascular disease, TSP-1 inhibits nitric oxide signaling via the CD47 pathway, promoting platelet aggregation and thrombosis. In diabetic nephropathy, TSP-1-mediated TGF-β activation drives renal fibrosis. Therefore, TSP-1 serves as a crucial regulatory hub in the extracellular matrix, playing complex and important roles in vascular biology, immune regulation, and fibrotic diseases.

Picture

Western Blot

WB result of Thrombospondin 1 Recombinant Rabbit mAb
Primary antibody: Thrombospondin 1 Recombinant Rabbit mAb at 1/××× dilution
Lane 1: mouse platelet lysate 5 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 129 kDa
Observed MW: 140, 180 kDa

WB result of Thrombospondin 1 Recombinant Rabbit mAb
Primary antibody: Thrombospondin 1 Recombinant Rabbit mAb at 1/××× dilution
Lane 1: rat platelet lysate 5 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 129 kDa
Observed MW: 140, 180 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human bone marrow. Anti- Thrombospondin 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human spleen. Anti- Thrombospondin 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse bone marrow. Anti- Thrombospondin 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse spleen. Anti- Thrombospondin 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat bone marrow. Anti- Thrombospondin 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat spleen. Anti- Thrombospondin 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.