WB result of TGN46 Recombinant Rabbit mAb
Primary antibody: TGN46 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: A172 whole cell lysate 20 µg
Low expression control: HeLa whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 75-100 kDa
TGN46 Recombinant Rabbit mAb (S-3514-47)
TGN46 Recombinant Rabbit mAb (S-3514-47)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TGN46 |
| Synonyms | Trans-Golgi network integral membrane protein 2; Trans-Golgi network glycoprotein 46 (TGN38 homolog; hTGN46); Trans-Golgi network glycoprotein 48 (hTGN48); Trans-Golgi network glycoprotein 51 (hTGN51); Trans-Golgi network protein 2Imported; TGN51; TGOLN2 |
| Immunogen | Recombinant Protein |
| Location | Endosome, Cell membrane |
| Accession | O43493 |
| Clone Number | S-3514-47 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | HepG2, A549, A172 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
TGN46 (Trans-Golgi Network protein 46 kDa) is a type I integral membrane protein predominantly localized to the trans-Golgi network (TGN), where it functions as a critical component of the protein sorting and vesicular trafficking machinery. This 46-kilodalton protein features a large N-terminal lumenal domain containing multiple N-glycosylation sites, a single transmembrane helix, and a short C-terminal cytoplasmic tail harboring sorting signals—including tyrosine-based motifs (YXXΦ)—that facilitate its interaction with adaptor protein complexes (AP-1 and AP-2) to regulate cargo selection and vesicle formation. TGN46 serves as a widely used marker for the TGN in immunofluorescence and cell biology studies, and plays essential roles in maintaining TGN structure, recycling mannose-6-phosphate receptors (MPRs) between endosomes and the Golgi, and sorting lysosomal enzymes into clathrin-coated vesicles destined for late endosomes. Its dynamic cycling between the TGN, endosomes, and the plasma membrane—mediated by phosphorylation-dependent conformational changes and interactions with various coat proteins—underscores its significance in secretory and endocytic pathway regulation, making it indispensable for proper cellular homeostasis and protein trafficking fidelity.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-TGN46 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-TGN46 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-TGN46 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-TGN46 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-TGN46 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in A549 cells. Anti-TGN46 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
