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TGFBI / BIGH3 Mouse mAb

TGFBI / BIGH3 Mouse mAb

Catalog Number: S0B60280 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Mouse
Antigen TGFBI / BIGH3
Synonyms Transforming growth factor-beta-induced protein ig-h3; Kerato-epithelin; RGD-containing collagen-associated protein (RGD-CAP); BIGH3; TGFBI
Location Secreted
Accession Q15582
Antibody Type Mouse mAb
Isotype IgG2a
Application WB, IHC-P
Reactivity Hu, Ms, Rt
Positive Sample U-87 MG, mouse liver, rat eye
Purification Protein A
Concentration 1.3 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Hu, Ms, Rt
IHC-P 1:500 Hu

Background

TGFBI (Transforming Growth Factor Beta-Induced), also known as βig-H3, is an extracellular matrix protein that plays a critical role in cell adhesion, bone formation, and wound healing. Clinically, it is best known for its association with hereditary corneal dystrophies, where mutations lead to abnormal protein accumulation in the eye. In cancer biology, TGFBI exhibits a context-dependent dual role: it can act as a tumor suppressor in early stages by enhancing chemotherapy sensitivity, but often functions as a tumor promoter in established tumors by facilitating metastasis and cell survival.

Picture

Western Blot

WB result of TGFBI / BIGH3 Mouse mAb
Primary antibody: TGFBI / BIGH3 Mouse mAb at 1/1000 dilution
Lane 1: U-87 MG hole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 75 kDa
Observed MW: 68 kDa
This blot was developed with high sensitivity substrate

WB result of TGFBI / BIGH3 Mouse mAb
Primary antibody: TGFBI / BIGH3 Mouse mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-Mouse (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 75 kDa
Observed MW: 55 kDa
This blot was developed with high sensitivity substrate

WB result of TGFBI / BIGH3 Mouse mAb
Primary antibody: TGFBI / BIGH3 Mouse mAb at 1/1000 dilution
Lane 1: rat eye lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 75 kDa
Observed MW: 52 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human kidney. Anti-TGFBI / BIGH3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-TGFBI / BIGH3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-TGFBI / BIGH3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.