WB result of Teraspanin-33 Recombinant Rabbit mAb
Primary antibody: Teraspanin-33 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled Raji whole cell lysate 20 µg
Lane 2: unboiled Ramos whole cell lysate 20 µg
Lane 3: unboiled Daudi whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 35 kDa
Teraspanin-33 Recombinant Rabbit mAb (S-1953-44)
Teraspanin-33 Recombinant Rabbit mAb (S-1953-44)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | teraspanin-33 |
| Synonyms | Tspan-33; Penumbra (hPen); Proerythroblast new membrane; PEN; TSPAN33 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cell membrane |
| Accession | Q86UF1 |
| Clone Number | S-1953-44 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Positive Sample | Raji, Ramos, Daudi |
| Predicted Reactivity | Bv |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu |
Background
Tetraspanin-33 (Tspan-33, encoded by TSPAN33, also called Penumbra) is a four-transmembrane-domain protein of the tetraspanin superfamily that is virtually absent from resting B cells but strongly induced upon activation, localizes to membrane microvilli, the Golgi and extracellular vesicles, and functions as a key regulator of human B-lymphocyte endocytosis, adhesion and migration by modulating plasma-membrane tension and cytoskeletal dynamics; it is furthermore expressed in several lymphomas and autoimmune diseases, qualifying it as a potential diagnostic biomarker and therapeutic target for malignant B cells.
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Western Blot
