WB result of TDG Recombinant Rabbit mAb
Primary antibody: TDG Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: HCT 116 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 50, 60 kDa
This blot was developed with high sensitivity substrate
TDG Recombinant Rabbit mAb (S-3111-16)
TDG Recombinant Rabbit mAb (S-3111-16)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TDG |
| Synonyms | G/T mismatch-specific thymine DNA glycosylase; Thymine-DNA glycosylase (hTDG) |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q13569 |
| Clone Number | S-3111-16 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | 293T, HeLa, K562, Jurkat, HCT 116 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:50 | Hu |
Background
TDG (thymine DNA glycosylase) is a 410-residue nuclear enzyme that belongs to the uracil DNA glycosylase (UDG) superfamily and acts as a key guardian of epigenetic stability by excising thymine, 5-formylcytosine, 5-carboxylcytosine and other deaminated or oxidized bases from G•T, G•U or G•5fC/5caC mispairs in double-stranded DNA, thereby initiating base-excision repair (BER), reshaping CpG methylation landscapes and preventing C→T transition mutations and transcriptional misregulation in embryonic stem cells and somatic tissues.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-TDG antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-TDG antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-TDG antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-TDG antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-TDG antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
