WB result of TBR2/Eomes Recombinant Rabbit mAb
Primary antibody: TBR2/Eomes Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: NK-92 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 73 kDa
Observed MW: 55-85 kDa
TBR2/Eomes Recombinant Rabbit mAb (SDT-1101-231)
TBR2/Eomes Recombinant Rabbit mAb (SDT-1101-231)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TBR2/Eomes |
| Synonyms | Eomesodermin homolog; T-box brain protein 2 (T-brain-2; TBR-2); EOMES |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | O95936 |
| Clone Number | SDT-1101-231 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | NK-92, mouse E14 embryo brain, rat E14 embryo brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| IF | 1:500 | Ms, Rt |
Background
TBR2/Eomes is a T-box transcription factor that plays crucial roles in various developmental processes. It is preferentially enriched in intermediate progenitors and supports cortical neurogenesis expansion. In the developing cerebral cortex, it is specifically expressed in intermediate progenitors (IPs), which are derived from radial glia progenitors and generate glutamatergic projection neurons. TBR2/Eomes is essential for the differentiation and subtype specification of these neurons, and it regulates the transcriptome by activating or repressing hundreds of direct target genes. Additionally, TBR2/Eomes is involved in the formation and maintenance of certain retinal ganglion cells. It also plays a role in the maintenance of the trophectoderm lineage by sustaining chromatin accessibility and promoting gene regulatory networks.
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Picture
Western Blot
WB result of TBR2/Eomes Recombinant Rabbit mAb
Primary antibody: TBR2/Eomes Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse E14 embryo brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 73 kDa
Observed MW: 60- 90 kDa
WB result of TBR2/Eomes Recombinant Rabbit mAb
Primary antibody: TBR2/Eomes Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat E14 embryo brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 73 kDa
Observed MW: 80, 90 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-TBR2/Eomes antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-TBR2/Eomes antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-TBR2/Eomes antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-TBR2/Eomes antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-TBR2/Eomes antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-TBR2/Eomes antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse E14 embryo. Anti-TBR2/Eomes antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat E14 embryo. Anti-TBR2/Eomes antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
