WB result of Tau (phospho T212/S214) Recombinant Rabbit mAb
Primary antibody: Tau (phospho T212/S214) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T transfected with myc-His-tag, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Lane 2: 293T transfected with myc-His-tag, then treated with 100 nM Calyculin A for 30 minutes, then treated with phosphatase whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 79 kDa
Observed MW: 35~100 kDa
Tau (phospho T212/S214) Recombinant Rabbit mAb (S-1538-74)
Tau (phospho T212/S214) Recombinant Rabbit mAb (S-1538-74)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Tau (phospho T212/S214) |
| Synonyms | Microtubule-associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau (PHF-tau); MAPT; MAPTL; MTBT1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P10636-8 |
| Clone Number | S-1538-74 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Theoretically reacts with most common species |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms, Rt |
Background
Tau (phospho T212/S214) refers to the phosphorylation of the tau protein at threonine 212 and serine 214 residues. Phosphorylation at T212 and S214 is associated with the formation of neurofibrillary tangles (NFTs), a hallmark of Alzheimer's disease and other tauopathies. Phosphorylation at T212 and S214 affects the interaction of Tau with DNA and nucleosomes. This modification can reduce its DNA binding affinity and disrupt its phase separation with 12mer arrays, which may have implications for tau's biological role in the nucleus. The levels of phosphorylated tau at T212/S214 increase with healthy aging, and Alzheimer's progression correlates with an increase of nuclear tau phosphorylated at S202/T205 (AT8 antibody epitope), culminating with the exit of tau from the nucleus.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of Tau (phospho T212/S214) Recombinant Rabbit mAb
Lane1: Tau (Thr212&Ser214) phospho peptide
Lane2: Tau (Thr212) phospho peptide
Lane3: Tau (Ser214) phospho peptide
Lane4: Tau (Thr212&Ser214) unmodified peptide
Primary antibody: Tau (phospho T212/S214) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
